Day 1

​

09:00 – 09:10 – Welcome & Opening Remarks (10 min)

 

Session 1: Genetic Screening for Biological Discovery

Session chair: Jonathan Schmid-Burgk

​

09:10 – 10:40 (90 min)

• Thijn Brummelkamp, Netherlands Cancer Institute, The Netherlands (25 min)

Genetics in haploid cells to identify ‘Alternative Pathways’

• Silvia Domcke, University of Zurich, Switzerland (25 min)

Overcoming confounders in organoid-based CRISPR screens​

• Kyla Foster, University of California San Francisco, United States (5 min)

High-content single-nuclei perturbation profiling links CRISPRi dependencies to regulatory architecture in meningioma

• Luke Gilbert, Arc Institute & University of California San Francisco, United States (25 min)

Scalable probe-based single-cell transcriptional profiling for virtual cell perturbation mapping and synthetic biology phenotyping

• Jelle Jacobs, Leuven Institute for Single Cell Omics, Belgium (5 min)

LipoGrid: A multi-omics high throughput screening platform for the systematic dissection of lipid metabolism via genetic perturbations

• Werner Schenkel, BD Biosciences (5 min)

​

10:40 – 11:15 – Morning Coffee Break (35 min)

 

Session 2: CRISPR Screening with Single-Cell Readout​

Session chair: Luke Gilbert

​

11:15 – 12:45 (90 min)

• Christoph Bock, Center for Molecular Medicine & Medical University Vienna, Austria (25 min)

Programmed cells? Single-cell biology and cell engineering for immunity and cancer

• Laura Kida, Memorial Sloan Kettering Cancer Center, United States (5 min)

Translational perturbation screening in single-cells via multi-omic tRNA-sequencing

• Thomas Norman, Memorial Sloan Kettering Cancer Center, United States (25 min)

Scaling perturbations: beyond genome scale CRISPR screens

• Heonseok Kim, Hanyang University, South Korea (5 min)

Simultaneous single-cell measurement of engineered mutations, transcript isoforms, and transcriptomic phenotypes

• Ci Chu, Xaira Therapeutics, United States (25 min)

Towards the virtual cell: Bridging genome-scale Perturb-seq data and causal AI models

• Peter Smibert, 10x Genomics (5 min)

​​

12:45 – 14:00 – Lunch Break (75 min)

 

Session 3: Computational Methods and Modeling

​Session chair: Wei Li​​​

​

14:00 – 15:30 (90 min)

• Rahul Satija, New York Genome Center, United States (25 min)

• Alex Ng, GC Therapeutics, United States (5 min)

AI-guided cell fate programming trained by systematic TFome™-wide screening

• Leopold Parts, Wellcome Sanger Institute, United Kingdom (25 min)

Screening structural variation in the human genome

• Mo Lotfollahi, Welcome Sanger Institute, United Kingdom (25 min)

• Rea Dabelic, Illumina (5 min)

• Milad Dagher, NOMIC Bio (5 min)

​​

15:30 – 16:00 – Afternoon Coffee Break (30 min)

 

Session 4: Advanced Models – iPSC & in vivo Screening

​Session chair: Thomas Norman​

 

16:00 – 17:25 (85 min)

• Xin Jin, The Scripps Research Institute, United States (25 min)

Toward a functional brain genome

• Lesly Calderon, Helmholtz Munich & Technical University of Munich, Germany (5 min)

In vivo CRISPR/Cas9 screens reveal novel regulators of B cell activation and differentiation into effector B cells

• Andreas E. Moor, ETH Zurich, Switzerland (25 min)

Perturbation of cellular interactions in metastasis

• Koen Oost, Friedrich Miescher Institute for Biomedical Research, Switzerland (25 min)

From cells to tissues: Dissecting regenerative dynamics using optical pooled screening in human colonic organoids​

• Sejla Salic-Hainzl, bit.bio discovery (5 min)

​​

17:25 – 19:00 – Poster Session & Networking

 

18:30 – Dinner

 

Day 2

 

Session 5: CRISPR Screening for Cancer Dependencies

​Session chair: Christoph Bock​

​

09:00 – 10:30 (90 min)

• Mathew Garnett, Wellcome Sanger Institute, United Kingdom (25 min)

A tumour-derived organoid biobank maps cancer gene dependencies

• Katharina Mikulik, German Cancer Research Center, Germany (5 min)

Uncovering the time- and context-dependent origins of congenital heart disease through CRISPR perturbation screening in organoids

• Francesco Iorio, Human Technopole, Italy (25 min)

Mining existing and newly designed CRISPR screens to map genomic, transcriptomic and phenotypic contexts of cancer dependencies

• Johannes Zuber, Research Institute of Molecular Pathology, Austria (25 min)

Defining cancer dependencies in vivo

• Christian Hundsrucker, Microsynth (5 min)

• Jennifer Stott, IDT (5 min)

 

10:30 – 11:00 – Morning Coffee Break (30 min)

 

Session 6: CRISPR Screening in Primary Human Cells

​Session chair: Xin Jin

​

11:00 – 12:30 (90 min)

• Tilmann Bürckstümmer, Myllia Biotechnology, Austria (25 min)

Single-cell CRISPR screens in primary human myeloid cells

• Radu Rapiteanu, GSK, United Kingdom (25 min)

Target discovery through multimodal single cell CRISPR screens in primary immune cells

• Jingwen Ding, University of California San Francisco, United States (5 min)

Dissecting gene regulatory networks governing human cortical cell fate​

• Chong Li, Chinese Institute for Brain Research, China (5 min)

Decoding neurodevelopmental disorders with single-cell functional genomics in brain organoids

• Jake Taylor-King, Relation Therapeutics, United Kingdom (25 min)

Extracting cytokine perturbation signatures from full-length spatial transcriptomics using biophysical modelling

• Kristin Atze, Lonza (5 min)

​​

12:30 – 14:00 – Lunch Break (90 minutes)

 

Session 7: Imaging-based CRISPR Screens

​Session chair: Mathew Garnett 

​

14:00 – 15:05 (65 min)

• Samouil Farhi, Broad Institute, United States (25 min)

Simultaneous CRISPR screening and spatial -omics

• Russell Walton, Broad Institute of MIT and Harvard, United States (5 min)

Multi-modal combinatorial CRISPR screens with CROPseq-multi for high-content phenotyping of genetic interactions at scale​

• Jonathan Schmid-Burgk, University of Bonn, Germany (25 min)

​Pooled experiments for studying sub-cellular signal processing

• Wilfred Van IJcken, Element Biosciences (5 min)

• Josh Croteau, Revvity (5 min)

 

15:05 – 15:35 – Afternoon Coffee Break (30 min)

 

Session 8: Foundation Models of Human Cells

Session chair: Jake Taylor-King

​

15:35 – 16:55 (80 min)

• Wei Li, University of Maryland, United States (25 min)

Understanding dosage responses and building AI prediction models from single-cell perturbation datasets​

• Jin Chen, Altos Labs, United States (5 min)

Large-scale Perturb-seq in primary human cells reveals disease-reversal programs

• Yusuf Roohani, Arc Institute, United States (25 min)

Engineering cell state using artificial intelligence​

• Bo Wang, Xaira Therapeutics, United States (25 min)

 

16:55 – 17:10 – Announcement of the Winners of Myllia's Virtual Cell Challenge called "Echoes of Silenced Genes"

​

17:10 – 17:25 – Closing Remarks (15 min)